Process of Cellular Division in Escherichia coli : Physiological Study on Thermosensitive Mutants Defective in Cell Division
نویسندگان
چکیده
منابع مشابه
Genetical and physiological studies on a thermosensitive mutant of Escherichia coli defective in cell division.
A new temperature-sensitive mutant of E. coli, defective in cell division, was isolated after selection for tolerance to colicin E2. The mutant strain, ASHI24, growing in either minimal or complex medium, commences filament formation immediately upon shift to high temperature. High densities of bacteria or the presence of 0-44 M-sucrose prevents filament formation at 42 degrees C and division c...
متن کاملMutant of Escherichia coli with thermosensitive protein in the process of cellular division.
A new thermosensitive mutant of Escherichia coli deficient in cell division was isolated by means of membrane filtration after nitrosoguanidine mutagenesis. The mutant cells grow normally at 30 C but stop dividing immediately after shift to 42 C, resulting in multinucleated filaments lacking septa. The number of colony-forming units does not decrease for at least 6 hr at 42 C. The maximum lengt...
متن کاملLow-temperature conditional cell division mutants of Escherichia coli.
Fifteen low-temperature conditional division mutants of Escherichia coli K-12 was isolated. They grew normally at 39 degrees C but formed filaments at 30 degrees C. All exhibited a coordinated burst of cell division when the filaments were shifted to the permissive temperature (39 degrees C). None of the various agents that stimulate cell division in other mutant systems (salt, sucrose, ethanol...
متن کاملDefective in Cell Division
Thermosensitive fts mutants of Escherichia coli belonging to seven previously identified genetic classes (ftsA to ftsG) were studied from a physiological standpoint. These mutants immediately stopped dividing and formed multinucleated filaments when the temperature was shifted to 41 C. Macromolecular syntheses (deoxyribonucleic acid), ribonucleic acid, cell mass, and murein) continued exponenti...
متن کاملEffects of chromosome underreplication on cell division in Escherichia coli.
The key processes of the bacterial cell cycle are controlled and coordinated to match cellular mass growth. We have studied the coordination between replication and cell division by using a temperature-controlled Escherichia coli intR1 strain. In this strain, the initiation time for chromosome replication can be displaced to later (underreplication) or earlier (overreplication) times in the cel...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1973
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.116.1.314-322.1973